Journal: Signal Transduction and Targeted Therapy

Article Title: Prostaglandin E 2 -driven dedifferentiation of Schwann cells leads to perineural invasion in pancreatic ductal adenocarcinoma

doi: 10.1038/s41392-026-02648-x

Figure Lengend Snippet: SCs are enriched in the PNI-positive region. a Schematic diagram of the workflow; some of the graphical elements were created wtith BioGDP. b Division of perineural invasion regions (PNI-region: PNI, PNI-near, PNI-mid, PNI-away) and nonperineural invasion regions (NPNI: NPNI, NPNI-near, NPNI-mid, NPNI-away) in representative spatial transcriptomic images of PDAC PNI samples, with corresponding representative HE-stained images of spot points (right) (n = 4). N: Nerve, T: Tumor. Scale bar: 500 μm. c GSVA of the PNI region and the NPNI region. d Correlations between different cell types and the expression levels of marker genes. e Cell type distribution scatter plot based on the UMAP dimensionality reduction algorithm; f UMAP plot of SC subsets in pancreatic cancer tissues. g Feature score of nomyelinating SCs at the single-cell level in each of the two SC subsets ( p = 0.025). h Representative images of RCTD single-cell mapping results based on PNI regions and NPNI regions (n = 4); i Stacked bar charts of different PNI regions and NPNI regions, showing the proportions of various cell types in different samples. j , k Representative mIHC images of human normal pancreatic tissues, PDAC-PNI- tissues, and PDAC-PNI+ tissues stained with PGP9.5, SOX2, c-Jun, and MUC1 antibodies. Scale bar: 100 μm (left), 50 μm (right). The ratios of SOX2+ and c-Jun+ positive cells in normal pancreatic tissues, PDAC-PNI- tissues, and PDAC-PNI+ tissues ( n = 5). All the results are presented as the means ± SDs. Each data point in the bar graphs represents an individual sample. Statistical significance was determined by a two-tailed unpaired t test (Fig. 1g) and one-way analysis of variance followed by Tukey’s HSD post hoc test (Fig. 1j). **** p < 0.0001

Article Snippet: For mIHC staining, formalin-fixed sections were stained with antibodies against PGP9.5 (1:200, Abways, CY6722), MUC1 (1:200, BOSTER, BM4548), SOX2 (1:200, Diagbio, db16528), c-Jun (1:200, Selleck, F0168), and p75NTR (1:200, ABclonal, A11169) according to the manufacturer’s instructions for the multicolor immunofluorescence kit (Zolgene, PR-004507) and imaged with a KFbio Pathology Imaging System (KF-FL-005).

Techniques: Staining, Expressing, Marker, Single Cell, Two Tailed Test

Journal: Signal Transduction and Targeted Therapy

Article Title: Prostaglandin E 2 -driven dedifferentiation of Schwann cells leads to perineural invasion in pancreatic ductal adenocarcinoma

doi: 10.1038/s41392-026-02648-x

Figure Lengend Snippet: Elevation of PTGES/PGE 2 in the coculture system is correlated with PNI. a Venn diagram showing the intersection of multiomics data identifying PTGES as a key gene in PNI. b Expression of PTGES genes via spatial transcriptome analysis. Scale bar: 500 μm ( n = 4). c Violin plot showing the expression of PTGES in various PNI regions and NPNI regions via spatial transcriptomics ( n = 4). d Representative mIHC images of human normal pancreatic tissues, PDAC-PNI- tissues, and PDAC-PNI+ tissues stained with PGP9.5, p75NTR and MUC1 antibodies ( n = 3). Scale bar: 100 μm (left), 50 μm (right). e IHC staining of PTGES in human normal pancreatic tissues, PDAC-PNI- tissues, and PDAC-PNI+ tissues ( n = 9), N: nerve, T: tumor. Scale bar: 100 μm. l H-scores of PTGES. f , i Western blotting was performed to detect the protein expression level of PTGES in human normal pancreatic tissues and PDAC tissues ( n = 3), N: normal tissue, T: tumor tissue. g , j Western blotting was used to determine the protein expression level of PTGES in PANC-1 and BxPC-3 cells after coculture with RSC96 cells ( n = 3). h , k Western blotting was conducted to measure the protein expression level of PTGES in PANC-1 and BxPC-3 cells after coculture with sNF96.2 cells ( n = 3). m ELISA was used to detect the concentration of PGE 2 in the culture medium ( n = 3), and some of the graphical elements were created wtith Figdraw. All the results are presented as the means ± SDs. Each data point in the graphs represents an individual sample. Statistical significance was determined via two-tailed unpaired t tests (for Fig. 4i–k), one-way analysis of variance followed by Tukey’s HSD post hoc test (for Fig. 4m) and the Kruskal‒Wallis H test followed by Dunn’s multiple comparisons test (for Fig. 4l). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: For mIHC staining, formalin-fixed sections were stained with antibodies against PGP9.5 (1:200, Abways, CY6722), MUC1 (1:200, BOSTER, BM4548), SOX2 (1:200, Diagbio, db16528), c-Jun (1:200, Selleck, F0168), and p75NTR (1:200, ABclonal, A11169) according to the manufacturer’s instructions for the multicolor immunofluorescence kit (Zolgene, PR-004507) and imaged with a KFbio Pathology Imaging System (KF-FL-005).

Techniques: Expressing, Spatial Transcriptomics, Staining, Immunohistochemistry, Western Blot, Enzyme-linked Immunosorbent Assay, Concentration Assay, Two Tailed Test